Course Description
This course provides a step-by-step guideline of how to freeze semen from stallions and how frozen semen is stored, transported and subsequently thawed for insemination into mares. The goal is to furnish stallion owners, managers, technicians and veterinarians with both general principles and detailed techniques regarding semen freezing in stallions.
Lectures will include the advantages and disadvantages of frozen semen, principles of cryopreservation, importance of a test freeze, laboratory equipment and supplies required to freeze semen, detailed instructions on how to process and freeze semen, how to evaluate the quality of a frozen semen sample, methods to store and transport frozen semen, optimal management strategies for mares being bred with frozen semen and procedures for insemination of mares with frozen semen. In addition, a video overview of the entire process from initial evaluation of a stallion semen sample through the freezing and storage process is provided. An ‘appendix of videos’ describing important background techniques such as how to determine sperm concentration using a hemacytometer and a NucleoCounter®, how to centrifuge stallion semen and safety procedures when working with liquid nitrogen are also provided. Finally, a series of charts, worksheets, how-to guides, data logs and protocols are included in the course material. There is no prerequisite for this course.
Category
Equine
Instructors
Dr. Patrick McCue
Paula Moffett
Dr. James K. Graham
Race Approved CE
5.0 Hours
Cost
$250.00
Course Type
Online
Agenda
Course Lectures
1. Introduction – Stallion Frozen Semen
2. Principles of Freezing
3. Test Freeze – A Standard Recommendation
4. Laboratory equipment and supplies
5. Semen Collection and Evaluation
6. Equine semen freezing – Part 1
7. Equine semen freezing – Part 2
8. Post Thaw Evaluation of Frozen Semen
9. Handling Long-term Storage and Shipping of Frozen Semen
10. Mare Management for Breeding with Frozen Semen
11. Insemination of Mares with Frozen Semen
12. Video demonstration of the Entire Freeze Process
Appendix Lectures
1. Hemacytometer Techniques
2. NucleoCounter Technique
3. Centrifugation Techniques
4. Safety When Working With Liquid Nitrogen
Instructors
Dr. Patrick McCue
DVM, PhD, DACT
Paula Moffett
BS, MS, Research Associate
Dr. James K. Graham
Ph.D., Professor