Stallion Frozen Semen Online Course


Course Welcome from one of the course instructors, Paula Moffett, BS, MS

Course Description
This course provides a step-by-step guideline of how to freeze semen from stallions and how frozen semen is stored, transported and subsequently thawed for insemination into mares. The goal is to furnish stallion owners, managers, technicians and veterinarians with both general principles and detailed techniques regarding semen freezing in stallions.

Lectures will include the advantages and disadvantages of frozen semen, principles of cryopreservation, importance of a test freeze, laboratory equipment and supplies required to freeze semen, detailed instructions on how to process and freeze semen, how to evaluate the quality of a frozen semen sample, methods to store and transport frozen semen, optimal management strategies for mares being bred with frozen semen and procedures for insemination of mares with frozen semen. In addition, a video overview of the entire process from initial evaluation of a stallion semen sample through the freezing and storage process is provided. An ‘appendix of videos’ describing important background techniques such as how to determine sperm concentration using a hemacytometer and a NucleoCounter®, how to centrifuge stallion semen and safety procedures when working with liquid nitrogen are also provided. Finally, a series of charts, worksheets, how-to guides, data logs and protocols are included in the course material. There is no prerequisite for this course.

Course Lectures
1. Introduction – Stallion Frozen Semen
2. Principles of Freezing
3. Test Freeze – A Standard Recommendation
4. Laboratory equipment and supplies
5. Semen Collection and Evaluation
6. Equine semen freezing – Part 1
7. Equine semen freezing – Part 2
8. Post Thaw Evaluation of Frozen Semen
9. Handling Long-term Storage and Shipping of Frozen Semen
10. Mare Management for Breeding with Frozen Semen
11. Insemination of Mares with Frozen Semen
12. Video demonstration of the Entire Freeze Process

Appendix Lectures
1. Hemacytometer Techniques
2. NucleoCounter Technique
3. Centrifugation Techniques
4. Safety When Working With Liquid Nitrogen

Paula Moffett, BS, MS, Research Associate
Paula received a B.S. at Oklahoma State University in 2000. She later completed a M.S. at Colorado State University in 2008. Her Master’s research was based on cryopreservation of equine sperm and has continued efforts to improve the success of frozen semen by utilizing a dual cryoprotectant. She has remained at Colorado State University as a research associate at the Equine Reproduction Laboratory in the Department of Biomedical Sciences in the College of Veterinary Medicine and Biomedical Sciences. She, also, coordinates the stallion program at the Equine Reproduction Laboratory which consists of research, teaching and commercial stallion semen collections and sperm cryopreservation. Paula is also actively involved in day to day client interaction with emphasis on stallion handling and behavior. In addition, she provides continuing education to horse owners, veterinarians and students and helps with ongoing research. Research interests: cryopreservation of equine sperm, salvaging epididymal sperm, refreezing frozen sperm for ICSI use.

James K. Graham, Ph.D., Professor
Dr. Graham received a B.S. at the University of Minnesota and Ph.D. at Cornell University in Reproductive Physiology. He received postdoctoral training at Michigan State University and the Pennsylvania State University before joining the Animal Reproduction Laboratory at Colorado State University in 1990. He teaches undergraduate, graduate and veterinary courses and is currently coordinated a new Master’s degree program specializing in assisted reproductive techniques. He has concentrated his research efforts in improving semen cryopreservation and semen analysis, utilizing many different animal models, including equine.

Patrick McCue, DVM, PhD, DACT
Dr. Patrick McCue graduated from veterinary school at the University of California, Davis, in 1986. He subsequently completed an internship in Large Animal Medicine and Surgery at the University of Pennsylvania and a residency in Equine Reproduction at the University of California, Davis. He became a Diplomate of the American College of Theriogenologists in 1991 and received a PhD in Comparative Pathology, with an emphasis on reproductive endocrinology and ovarian pathology in the mare, from UC-Davis in 1992. Dr. McCue joined the faculty at Colorado State University in 1994 where he is the Iron Rose Ranch Professor of Equine Theriogenology.

Educational Partners

Coyote Rock Ranch

Clarke Butte Ranch

Course Sponsors

Animal Reproduction Systems

Botupharma USA



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